Phagedisplay相关论文
Selection of porcine reproductive and respiratory syndrome virus mimotopes of a combinatorial librar
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) occurs worldwide and causes major economic losses in the swi......
Selection and characterization of single-chain recombinant antibodies against cyprinid herpesvirus 2
Antibody-displaying phage was selected in four panning rounds against cyprinid herpesvirus 2(CyHV-2)by phage display app......
Avian hepatitis E virus(HEV)is related genetically and antigenically to swine HEV and their capsid protein share common ......
Specific detection of various tumor types remains crucial for designing effective treatment strategies. We demonstrate p......
Background The biological behaviors of residual hepatoma cells after transarterial embolization therapy, which exist in ......
After embolization therapy,residual hepatoma cells exist in a hypoxic or even anaerobic tumor microenvironment....
In the mammalian immune system,hosts generate a wide variety of antibodies to target foreign molecules.Protein:protein a......
Identification of a novel Nogo-66 receptor-binding peptide that neutralize Nogo-66 inhibition of the
A Nogo-A to Nogo-66 receptor (NgR) has been well known to contribute to the inhibition of the neuriteregeneration of adu......
Background: Streptavidin is widely used in biological experiment owing to its highly specific, stable and tight affinity......
The recent Middle East respiratory syndrome coronavirus(MERS-CoV),Ebola and Zika virus outbreaks exemplify the continued......
Biocompatible chemical protein cleavage methods have been long-sought to replace enzymatic cleavages,but have yet to be ......
Phage display for peptide evolution suffered from amplification bias and incompatible throughputs between screening and ......
Transferrin receptor(CD71 or TFRC)is an attractive target for cancer therapy.However,high level of endogenic transferrin......
Phage Display技术是将外源蛋白通过与丝状噬菌体外壳蛋白融合而将外源蛋白表达于噬菌体颗粒的表面。该技术已被应用于噬菌体抗体......
期刊
Development and Application of an Immunoassay for Detection of Cd Based on Specific Nano-body Agains
In this study,p-SCN-Bn-DTPA was chosen as a bifunctional chelator to facilitate the synthesis of antigens for Cd,and......
噬菌体短肽库是将随机合成的寡核苷酸序列通过与单链噬菌体外壳蛋白基因融合，从而将随机短短肽表达于噬菌体的表面，将体外随机化学合......
从人ＡＦＰ免疫小鼠脾细胞ｍＲＮＡ中扩增出全套抗体Ｖ区基因并随机拼接为ＳｃＦｖ，构建全套ＳｃＦｖ基因噬菌体呈现文库，经两轮ｐａｎｎｉｎｇ筛选富集，一次从９４个单个重组噬菌体克隆中......
Zinc transporter 8(ZnT8) is a major autoantigen and a predictive marker in type 1 diabetes(T1D). To investigate ZnT8-spe......
The blood brain barrier represents a formidable obstacle for the transport of most systemati-cally administered neurodia......
AIM: Passive immunotherapy using antibody against hepatitis B surface antigen (HBsAg) has been advocated in certain case......
Human scFv antibody fragments specific for hepatocellular carcinoma selected from a phage display li
AIM: To identify the scFv antibody fragments specific for hepatocellular carcinoma by biopanning from a large human naiv......
噬菌体展示技术是将高度多样性的多肽或蛋白展示于噬菌体衣壳蛋白表面的一项技术.这一技术将展示分子的基因型和表型联系在一起,极......
In Vivo Selection of Phage Sequences and Characterization of Peptide-specific Binding to Breast Canc
OBJECTIVE To screen specific polypeptide target binding to breast cancer xenografts in vivo from a phage-displayed pepti......
噬菌体体内展示技术的出现,使器官靶向性多肽的筛选、器官特异性血管分子作图成为可能.更值得注意的是,通过动物体内的筛选,鉴定出......
目的 利用噬菌体表面展示技术，从半合成人源化单链可变区抗体库中筛选、鉴定丙型肝炎病毒（ＨＣＶ）非结构蛋白ＮＳ４Ａ的单链可变区抗体（ＳｃＦｖ）及其编码基因......
人干扰素α１ｃ／８６ＤＡＢ环突变文库的构建及细胞筛选马学军胡荣１吕海２魏开坤张丽兰薛水星侯云德（中国预防医学科学院病毒学研究所，病毒基因工程国......
目的 筛选能结合酪氨酸蛋白激酶受体ＥｐｈＢ２的功能短肽。方法 ＰＣＲ扩增ＥｐｈＢ２的配体结合区，定向克隆到融合表达质粒载体ｐＲＳＥＴ Ａ中，阳性克隆经ＩＰＴＧ诱导表达融合......
目的：通过噬菌体展示技术筛选与肝癌细胞特异性结合抗体结合的抗体。方法用肝癌细胞系SMMC7721免疫小鼠，提取脾细胞总RNA，构建单链抗......
构建适用于外源性随机多肽的噬菌体展示载体，方法在引物中加入ＸｂａⅠ识别序列，将用该引物扩增的ＰＣＲ产物克隆到ｐＧＥＭ－Ｔ中，再将该片段插入ｐＣＡＮＴＡＢ５Ｅ中构建成噬菌......
作为抗体库筛选的一个有效方法,噬菌体表面呈现技术在单链抗体的研制中得到广泛的应用.以噬菌粒pCANTAB5E和pHB为基础,利用PCR和DN......
克隆和连接乳腺癌杂交瘤单克隆抗体的轻、重链可变区基因，构建该单链抗体的噬菌体显示系统，经严格的相关肿瘤细胞株的固相细胞正负筛......
成功地扩增了有机磷毒剂梭曼类似物１－羟基－１－对胺基苯基甲膦酸单频哪基醇酯与血蓝蛋白结合物免疫小鼠脾细胞中抗体重键和轻链基因片段，构......
